A Genetic System for Methanocaldococcus jannaschii: An Evolutionary Deeply Rooted Hyperthermophilic Methanarchaeon
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چکیده
منابع مشابه
Reactor-scale cultivation of the hyperthermophilic methanarchaeon Methanococcus jannaschii to high cell densities.
For the hyperthermophilic and barophilic methanarchaeon Methanococcus jannaschii, we have developed a medium and protocols for reactor-scale cultivation that improved the final cell yield per liter from approximately 0.5 to approximately 7.5 g of packed wet cells ( approximately 1.8 g dry cell mass) under autotrophic growth conditions and to approximately 8.5 g of packed wet cells ( approximate...
متن کاملMetal Cation Binding by the Hyperthermophilic Microorganism, Archaea Methanocaldococcus Jannaschii, and Its Effects on Silicification
A series of experiments was made to determine the capacity of an archaeal strain, Methanocaldococcus jannaschii to bind metals and to study the effects of metal-binding on the subsequent silicification of the microorganisms. The results showed that M. jannaschii can rapidly bind several metal cations (Fe 3+ , Ca 2+ , Pb 2+ , Zn 2+ , Cu 2+ ). Considering the lack of silicification of this strain...
متن کاملProtein-coding gene promoters in Methanocaldococcus (Methanococcus) jannaschii
Although Methanocaldococcus (Methanococcus) jannaschii was the first archaeon to have its genome sequenced, little is known about the promoters of its protein-coding genes. To expand our knowledge, we have experimentally identified 131 promoters for 107 protein-coding genes in this genome by mapping their transcription start sites. Compared to previously identified promoters, more than half of ...
متن کاملPalindromic repetitive DNA elements with coding potential in Methanocaldococcus jannaschii.
We have identified 141 novel palindromic repetitive elements in the genome of euryarchaeon Methanocaldococcus jannaschii. The total length of these elements is 14.3kb, which corresponds to 0.9% of the total genomic sequence and 6.3% of all extragenic regions. The elements can be divided into three groups (MJRE1-3) based on the sequence similarity. The low sequence identity within each of the gr...
متن کاملMethanocaldococcus jannaschii uses a modified mevalonate pathway for biosynthesis of isopentenyl diphosphate.
Archaea have been shown to produce isoprenoids from mevalonate; however, genome analysis has failed to identify several genes in the mevalonate pathway on the basis of sequence similarity. A predicted archaeal kinase, coded for by the MJ0044 gene, was associated with other mevalonate pathway genes in the archaea and was predicted to be the "missing" phosphomevalonate kinase. The MJ0044-derived ...
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2019
ISSN: 1664-302X
DOI: 10.3389/fmicb.2019.01256